› 8cvf

X-ray diffraction
1.53Å resolution

Structure of L289F Hyoscyamine 6-beta Hydroxylase in complex with vanadyl, succinate, and hyoscyamine

Released:
DOI: 10.2210/pdb8cvf/pdb
PDB entry 8cvf coloured by chain, front view.
PDB entry 8cvf coloured by chain, side view.
PDB entry 8cvf coloured by chain, top view.
Source organism: Atropa belladonna
Entry authors: Wenger EW, Boal AK, Bollinger JM, Krebs C

Function and Biology Details

Reaction catalysed: 
L-hyoscyamine + 2-oxoglutarate + O(2) = (6S)-hydroxyhyoscyamine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-578637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe2OG dioxygenase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 42.67 KDa
Source organism: Atropa belladonna
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XJ43 (Residues: 1-343; Coverage: 100%)
Gene names: AbH6H, h6h
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand EDO 2 x EDO
Ligand FMT 5 x FMT
Ligand HYO 1 x HYO
Ligand SIN 1 x SIN
Ligand V 1 x V
Ligand O 1 x O
Ligand SR 2 x SR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 44.783Å b: 79.901Å c: 55.675Å
α: 90° β: 111.244° γ: 90°
R-values:
R R work R free
0.182 0.181 0.201
Expression system: Escherichia coli

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