› 8cvz

Electron Microscopy
3.52Å resolution

Human glycogenin-1 and glycogen synthase-1 complex in the apo ordered state

Released:
DOI: 10.2210/pdb8cvz/pdb
PDB entry 8cvz coloured by chain, front view.
PDB entry 8cvz coloured by chain, side view.
PDB entry 8cvz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural mechanism of human glycogen synthesis by the GYS1-GYG1 complex.
Fastman NM, Liu Y, Ramanan V, Merritt H, Ambing E, DePaoli-Roach AA, Roach PJ, Hurley TD, Mellem KT, Ullman JC, Green E, Morgans D, Tzitzilonis C
Cell Rep 40 111041 (2022)
PMID: 35793618
Related structures: EMD-27022

Function and Biology Details

Reactions catalysed: 
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin
UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146775 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glycogenin-1 Chains: E, F, G, H, I, J
Molecule details ›
Chains: E, F, G, H, I, J
Length: 352 amino acids
Theoretical weight: 39.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P46976 (Residues: 1-350; Coverage: 100%)
Gene names: GYG, GYG1
Sequence domains: Glycosyl transferase family 8
Glycogen [starch] synthase, muscle Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 634 amino acids
Theoretical weight: 72.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P13807 (Residues: 1-634; Coverage: 86%)
Gene names: GYS, GYS1
Sequence domains: Glycogen synthase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.52Å
Relevant EMDB volumes: EMD-27022
Expression system: Spodoptera frugiperda

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