› 8czl

X-ray diffraction
1.58Å resolution

Human LanCL1 bound to methyl glutathione (MeGSH)

Released:
DOI: 10.2210/pdb8czl/pdb
PDB entry 8czl coloured by chain, front view.
PDB entry 8czl coloured by chain, side view.
PDB entry 8czl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The mechanism of thia-Michael addition catalyzed by LanC enzymes.
Ongpipattanakul C, Liu S, Luo Y, Nair SK, van der Donk WA
OpenAccess logo Proc Natl Acad Sci U S A 120 e2217523120 (2023)
PMID: 36634136

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase LANCL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 47.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43813 (Residues: 1-399; Coverage: 100%)
Gene names: GPR69A, LANCL1
Sequence domains: Lanthionine synthetase C-like protein

Ligands and Environments


Cofactor: Ligand GSM 2 x GSM
1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 55.485Å b: 120.909Å c: 143.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Chemistry and biology of enzymes in protein glutathionylation.
Oppong et al. (2023)
2 other articles