› 8d33

Electron Microscopy
2.46Å resolution

Human mitochondrial DNA polymerase gamma ternary complex with GC basepair

Released:
DOI: 10.2210/pdb8d33/pdb
PDB entry 8d33 coloured by chain, front view.
PDB entry 8d33 coloured by chain, side view.
PDB entry 8d33 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Polγ coordinates DNA synthesis and proofreading to ensure mitochondrial genome integrity.
Park J, Herrmann GK, Mitchell PG, Sherman MB, Yin YW
Nat Struct Mol Biol (2023)
PMID: 37202477
Related structures: EMD-27154

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216609 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase subunit gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 1245 amino acids
Theoretical weight: 140.56 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P54098 (Residues: 1-1239; Coverage: 100%)
Gene names: MDP1, POLG, POLG1, POLGA
Sequence domains: DNA mitochondrial polymerase exonuclease domain
DNA polymerase subunit gamma-2 Chains: B, C
Molecule details ›
Chains: B, C
Length: 491 amino acids
Theoretical weight: 55.82 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9UHN1 (Residues: 1-485; Coverage: 100%)
Gene names: MTPOLB, POLG2
Sequence domains: Anticodon binding domain
DNA (5'-D(P*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*TP*AP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 24 nucleotides
Theoretical weight: 7.36 KDa
DNA (25-MER) Chain: T
Molecule details ›
Chain: T
Length: 28 nucleotides
Theoretical weight: 8.64 KDa

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand DCP 1 x DCP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.46Å
Relevant EMDB volumes: EMD-27154
Expression system: Insect cell expression vector pTIE1

Quick links

Citations

3 citation in other articles

Structural basis for processive daughter-strand synthesis and proofreading by the human leading-strand DNA polymerase Pol ε.
Roske et al. (2024)
2 more

3 mentions without citation

Polγ coordinates DNA synthesis and proofreading to ensure mitochondrial genome integrity.
Park et al. (2023)
2 more