› 8d3x

Electron Microscopy
4.1Å resolution

Human alpha3 Na+/K+-ATPase in its K+-occluded state

Released:
DOI: 10.2210/pdb8d3x/pdb
PDB entry 8d3x coloured by chain, front view.
PDB entry 8d3x coloured by chain, side view.
PDB entry 8d3x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for gating mechanism of the human sodium-potassium pump.
Nguyen PT, Deisl C, Fine M, Tippetts TS, Uchikawa E, Bai XC, Levine B
OpenAccess logo Nat Commun 13 5293 (2022)
PMID: 36075933
Related structures: EMD-27167

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Na(+)(Side 1) + K(+)(Side 2) = ADP + phosphate + Na(+)(Side 2) + K(+)(Side 1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-138330 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium/potassium-transporting ATPase subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 303 amino acids
Theoretical weight: 35.11 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector Flag-MCS-pcDNA3.1
UniProt:
  • Canonical: P05026 (Residues: 1-303; Coverage: 100%)
Gene names: ATP1B, ATP1B1
Sequence domains: Sodium / potassium ATPase beta chain
FXYD domain-containing ion transport regulator 6 Chain: G
Molecule details ›
Chain: G
Length: 98 amino acids
Theoretical weight: 10.86 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector Flag-MCS-pcDNA3.1
UniProt:
  • Canonical: Q9H0Q3 (Residues: 1-95; Coverage: 100%)
Gene names: FXYD6, UNQ521/PRO1056
Sequence domains: ATP1G1/PLM/MAT8 family
Sodium/potassium-transporting ATPase subunit alpha-3 Chain: A
Molecule details ›
Chain: A
Length: 1013 amino acids
Theoretical weight: 111.86 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector Flag-MCS-pcDNA3.1
UniProt:
  • Canonical: P13637 (Residues: 1-1013; Coverage: 100%)
Gene name: ATP1A3
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MF4 1 x MF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-27167
Expression system: Mammalian expression vector Flag-MCS-pcDNA3.1

Quick links

Citations

3 review citations

Electrostatic switch mechanisms of membrane protein trafficking and regulation.
Clarke RJ. (2023)
2 more

1 mention without citation

Structural basis for gating mechanism of the human sodium-potassium pump.
Nguyen et al. (2022)