› 8d74

Electron Microscopy
3.03Å resolution

Cryo-EM structure of human CNTF signaling complex: model containing the interaction core region

Released:
DOI: 10.2210/pdb8d74/pdb
PDB entry 8d74 coloured by chain, front view.
PDB entry 8d74 coloured by chain, side view.
PDB entry 8d74 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the assembly of gp130 family cytokine signaling complexes.
Zhou Y, Stevis PE, Cao J, Saotome K, Wu J, Glatman Zaretsky A, Haxhinasto S, Yancopoulos GD, Murphy AJ, Sleeman MW, Olson WC, Franklin MC
OpenAccess logo Sci Adv 9 eade4395 (2023)
PMID: 36930708
Related structures: EMD-27227

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-273466 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Interleukin-6 receptor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 625 amino acids
Theoretical weight: 71.23 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P40189 (Residues: 23-619; Coverage: 67%)
  • Best match: P40189-2 (Residues: 23-324)
Gene name: IL6ST
Sequence domains:
Ciliary neurotrophic factor Chain: D
Molecule details ›
Chain: D
Length: 214 amino acids
Theoretical weight: 24.7 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P26441 (Residues: 1-186; Coverage: 93%)
Gene name: CNTF
Sequence domains: Ciliary neurotrophic factor
Ciliary neurotrophic factor receptor subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 320 amino acids
Theoretical weight: 35.86 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P26992 (Residues: 23-342; Coverage: 91%)
Gene name: CNTFR
Sequence domains: Fibronectin type III domain
Leukemia inhibitory factor receptor Chain: B
Molecule details ›
Chain: B
Length: 817 amino acids
Theoretical weight: 92.83 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P42702 (Residues: 45-833; Coverage: 75%)
Gene name: LIFR
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.03Å
Relevant EMDB volumes: EMD-27227
Expression system: Cricetulus griseus

Quick links

Citations

9 citation in other articles

Human interleukin-12α and EBI3 are cytokines with anti-inflammatory functions.
Hildenbrand et al. (2023)
8 more

1 mention without citation

The JAK-STAT pathway: from structural biology to cytokine engineering.
Lv et al. (2024)