› 8d7e

Electron Microscopy
2.93Å resolution

Cryo-EM structure of human CNTFR alpha in complex with the Fab fragments of two antibodies

Released:
DOI: 10.2210/pdb8d7e/pdb
PDB entry 8d7e coloured by chain, front view.
PDB entry 8d7e coloured by chain, side view.
PDB entry 8d7e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the assembly of gp130 family cytokine signaling complexes.
Zhou Y, Stevis PE, Cao J, Saotome K, Wu J, Glatman Zaretsky A, Haxhinasto S, Yancopoulos GD, Murphy AJ, Sleeman MW, Olson WC, Franklin MC
OpenAccess logo Sci Adv 9 eade4395 (2023)
PMID: 36930708
Related structures: EMD-27228

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210928 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Ciliary neurotrophic factor receptor subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 348 amino acids
Theoretical weight: 39.17 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P26992 (Residues: 23-342; Coverage: 91%)
Gene name: CNTFR
Sequence domains: Fibronectin type III domain
H4H25311P2 antibody Fab fragment light chain Chain: G
Molecule details ›
Chain: G
Length: 215 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
H4H25311P2 antibody Fab fragment heavy chain Chain: F
Molecule details ›
Chain: F
Length: 217 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
REGN8938 antibody Fab fragment light chain Chain: E
Molecule details ›
Chain: E
Length: 215 amino acids
Theoretical weight: 23.44 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
REGN8938 antibody Fab fragment heavy chain Chain: D
Molecule details ›
Chain: D
Length: 220 amino acids
Theoretical weight: 23.92 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 8 x NAG
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-27228
Expression system: Cricetulus griseus

Quick links

Citations

4 citation in other articles

Exogenous l-fucose attenuates neuroinflammation induced by lipopolysaccharide.
Xu et al. (2023)
3 more