8d8n

Electron Microscopy
3.6Å resolution

gRAMP non-match PFS target RNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-100880 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
RAMP superfamily protein Chain: B
Molecule details ›
Chain: B
Length: 1717 amino acids
Theoretical weight: 197.22 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0B0EGF3 (Residues: 1-1717; Coverage: 100%)
Gene name: SCABRO_02597
Sequence domains: RAMP superfamily
RNA (35-MER) Chain: C
Molecule details ›
Chain: C
Length: 35 nucleotides
Theoretical weight: 11.08 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
RNA (5'-R(P*UP*CP*CP*GP*GP*GP*GP*CP*AP*GP*AP*AP*AP*AP*UP*UP*GP*GP*AP*CP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.82 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-27252
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'