› 8d96

Electron Microscopy
3.35Å resolution

Human DNA polymerase alpha/primase elongation complex I bound to primer/template

Released:
DOI: 10.2210/pdb8d96/pdb
PDB entry 8d96 coloured by chain, front view.
PDB entry 8d96 coloured by chain, side view.
PDB entry 8d96 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of human primosome elongation complexes.
He Q, Baranovskiy AG, Morstadt LM, Lisova AE, Babayeva ND, Lusk BL, Lim CJ, Tahirov TH
Nat Struct Mol Biol (2023)
PMID: 37069376
Related structures: EMD-27256

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216572 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (4 distinct):
DNA primase large subunit Chain: B
Molecule details ›
Chain: B
Length: 509 amino acids
Theoretical weight: 58.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P49643 (Residues: 1-509; Coverage: 100%)
Gene names: PRIM2, PRIM2A
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit
DNA polymerase alpha catalytic subunit Chain: C
Molecule details ›
Chain: C
Length: 1462 amino acids
Theoretical weight: 166.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P09884 (Residues: 1-1462; Coverage: 100%)
Gene names: POLA, POLA1
Sequence domains:
DNA (5'-D(*AP*TP*AP*AP*TP*GP*GP*TP*CP*GP*TP*GP*CP*CP*GP*CP*CP*AP*AP*TP*AP*A)-3') Chain: F
Molecule details ›
Chain: F
Length: 22 nucleotides
Theoretical weight: 6.76 KDa
DNA/RNA (5'-GTP)-R(P*GP*CP*GP*GP*CP*AP*CP*G)-D(P*AP*CP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 12 nucleotides
Theoretical weight: 3.98 KDa

Ligands and Environments

3 bound ligands:
Ligand SF4 1 x SF4
Ligand MG 1 x MG
Ligand DTP 1 x DTP
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
Resolution: 3.35Å
Relevant EMDB volumes: EMD-27256
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Models for human telomere C-strand fill-in by CST-PolĪ±-primase.
He et al. (2023)
1 more

1 mention without citation

Structures of human primosome elongation complexes.
He et al. (2023)