› 8d97

Electron Microscopy
3.8Å resolution

Apo gRAMP

Released:
DOI: 10.2210/pdb8d97/pdb
PDB entry 8d97 coloured by chain, front view.
PDB entry 8d97 coloured by chain, side view.
PDB entry 8d97 coloured by chain, top view.
Source organism: Candidatus Scalindua brodae
Primary publication:
Craspase is a CRISPR RNA-guided, RNA-activated protease.
Hu C, van Beljouw SPB, Nam KH, Schuler G, Ding F, Cui Y, Rodríguez-Molina A, Haagsma AC, Valk M, Pabst M, Brouns SJJ, Ke A
Science eadd5064 (2022)
PMID: 36007061
Related structures: EMD-27257

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217908 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RAMP superfamily protein Chain: A
Molecule details ›
Chain: A
Length: 1600 amino acids
Theoretical weight: 184.35 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0B0EGF3 (Residues: 1-1688; Coverage: 93%)
Gene name: SCABRO_02597
Sequence domains: RAMP superfamily
RNA (42-MER) Chain: C
Molecule details ›
Chain: C
Length: 42 nucleotides
Theoretical weight: 13.3 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-27257
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

5 review citations

Discovery of Diverse CRISPR-Cas Systems and Expansion of the Genome Engineering Toolbox.
Koonin et al. (2023)
4 more