› 8dak

X-ray diffraction
1.5Å resolution

Crystal structure of the GDP-D-glycero-4-keto-d-lyxo-heptose-3-epimerase from Campylobacter jejuni, serotype HS:3

Released:
DOI: 10.2210/pdb8dak/pdb
PDB entry 8dak coloured by chain, front view.
PDB entry 8dak coloured by chain, side view.
PDB entry 8dak coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
C3- and C3/C5-Epimerases Required for the Biosynthesis of the Capsular Polysaccharides from Campylobacter jejuni.
Ghosh MK, Xiang DF, Thoden JB, Holden HM, Raushel FM
Biochemistry 61 2036-2048 (2022)
PMID: 36093987

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative nucleotide-sugar epimerase/dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.19 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2X702 (Residues: 1-181; Coverage: 100%)
Gene names: HS3.15, HS3/13/50.15
Sequence domains: dTDP-4-dehydrorhamnose 3,5-epimerase

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand CL 4 x CL
Ligand TMA 1 x TMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P212121
Unit cell:
a: 61.534Å b: 67.932Å c: 96.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Product Specificity of C4-Reductases in the Biosynthesis of GDP-6-Deoxy-Heptoses during Capsular Polysaccharide Formation in Campylobacter jejuni.
Ghosh et al. (2022)
4 more

1 mention without citation

C3- and C3/C5-Epimerases Required for the Biosynthesis of the Capsular Polysaccharides from Campylobacter jejuni.
Ghosh et al. (2022)