› 8dbt

Electron Microscopy
3.1Å resolution

E. coli ATP synthase imaged in 10mM MgATP State2 "down

Released:
DOI: 10.2210/pdb8dbt/pdb
PDB entry 8dbt coloured by chain, front view.
PDB entry 8dbt coloured by chain, side view.
PDB entry 8dbt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Changes within the central stalk of E. coli F1Fo ATP synthase observed after addition of ATP.
Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG
OpenAccess logo Commun Biol 6 26 (2023)
PMID: 36631659
Related structures: EMD-27306

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-531800 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
ATP synthase subunit alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 513 amino acids
Theoretical weight: 55.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABB0 (Residues: 1-513; Coverage: 100%)
Gene names: JW3712, atpA, b3734, papA, uncA
Sequence domains:
ATP synthase subunit beta Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 471 amino acids
Theoretical weight: 51.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABB4 (Residues: 1-460; Coverage: 100%)
Gene names: JW3710, atpD, b3732, papB, uncD
Sequence domains:
ATP synthase gamma chain Chain: G
Molecule details ›
Chain: G
Length: 287 amino acids
Theoretical weight: 31.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABA6 (Residues: 1-287; Coverage: 100%)
Gene names: JW3711, atpG, b3733, papC, uncG
Sequence domains: ATP synthase
ATP synthase epsilon chain Chain: H
Molecule details ›
Chain: H
Length: 139 amino acids
Theoretical weight: 15.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6E6 (Residues: 1-139; Coverage: 100%)
Gene names: JW3709, atpC, b3731, papG, uncC
Sequence domains:
ATP synthase subunit delta Chain: W
Molecule details ›
Chain: W
Length: 177 amino acids
Theoretical weight: 19.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABA4 (Residues: 1-177; Coverage: 100%)
Gene names: JW3713, atpH, b3735, papE, uncH
Sequence domains: ATP synthase delta (OSCP) subunit
ATP synthase subunit c Chains: I, J, L, M, N, O, P, Q, R, S
Molecule details ›
Chains: I, J, L, M, N, O, P, Q, R, S
Length: 79 amino acids
Theoretical weight: 8.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P68699 (Residues: 1-79; Coverage: 100%)
Gene names: JW3715, atpE, b3737, papH, uncE
Sequence domains: ATP synthase subunit C
ATP synthase subunit b Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 156 amino acids
Theoretical weight: 17.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABA0 (Residues: 1-156; Coverage: 100%)
Gene names: JW3714, atpF, b3736, papF, uncF
Sequence domains: ATP synthase B/B' CF(0)
ATP synthase subunit a Chain: a
Molecule details ›
Chain: a
Length: 271 amino acids
Theoretical weight: 30.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AB98 (Residues: 1-271; Coverage: 100%)
Gene names: JW3716, atpB, b3738, papD, uncB
Sequence domains: ATP synthase A chain

Ligands and Environments

3 bound ligands:
Ligand ATP 4 x ATP
Ligand MG 5 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-27306
Expression system: Escherichia coli

Quick links

Citations

1 review citation

F1·Fo ATP Synthase/ATPase: Contemporary View on Unidirectional Catalysis.
Zharova et al. (2023)
3 other articles

1 mention without citation

Mechanism of ADP-Inhibited ATP Hydrolysis in Single Proton-Pumping FoF1-ATP Synthase Trapped in Solution.
Pérez et al. (2023)