› 8dg4

Electron Microscopy
3.12Å resolution

Group A streptococcus Enolase K252A, K255A, K434A, K435A mutant

Released:
DOI: 10.2210/pdb8dg4/pdb
PDB entry 8dg4 coloured by chain, front view.
PDB entry 8dg4 coloured by chain, side view.
PDB entry 8dg4 coloured by chain, top view.
Source organism: Streptococcus sp. 'group A'
Primary publication:
High-Resolution Single-Particle Cryo-EM Hydrated Structure of Streptococcus pyogenes Enolase Offers Insights into Its Function as a Plasminogen Receptor.
Tjia-Fleck S, Readnour BM, Ayinuola YA, Castellino FJ
Biochemistry 62 735-746 (2023)
PMID: 36701429
Related structures: EMD-27407

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 436 amino acids
Theoretical weight: 47.31 KDa
Source organism: Streptococcus sp. 'group A'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69949 (Residues: 1-435; Coverage: 100%)
Gene names: M5005_Spy0556, SPy_0731, eno
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.12Å
Relevant EMDB volumes: EMD-27407
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Recruitment and Activation of Plasminogen by Bacteria-The Involvement in Chronic Infection Development.
Satala et al. (2023)
1 other article