› 8dg8

Electron Microscopy
3.62Å resolution

Cryo-EM Structure of HPIV3 prefusion F trimer in complex with 3x1 Fab

Released:
DOI: 10.2210/pdb8dg8/pdb
PDB entry 8dg8 coloured by chain, front view.
PDB entry 8dg8 coloured by chain, side view.
PDB entry 8dg8 coloured by chain, top view.
Source organisms:
Primary publication:
Cross-protective antibodies against common endemic respiratory viruses.
Cabán M, Rodarte JV, Bibby M, Gray MD, Taylor JJ, Pancera M, Boonyaratanakornkit J
OpenAccess logo Nat Commun 14 798 (2023)
PMID: 36781872
Related structures: EMD-27418

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227117 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fusion glycoprotein F1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 495 amino acids
Theoretical weight: 54.97 KDa
Source organism: Human parainfluenza 3 virus (strain NIH 47885)
Expression system: Homo sapiens
UniProt:
  • Canonical: P06828 (Residues: 19-484; Coverage: 89%)
Gene name: F
Sequence domains: Fusion glycoprotein F0
3x1 monoclonal antibody, VH region Chain: H
Molecule details ›
Chain: H
Length: 128 amino acids
Theoretical weight: 13.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
3x1 monoclonal antibody, VL region Chain: L
Molecule details ›
Chain: L
Length: 108 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 9 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.62Å
Relevant EMDB volumes: EMD-27418
Expression system: Homo sapiens

Quick links

Citations

3 citation in other articles

Single B cell transcriptomics identifies multiple isotypes of broadly neutralizing antibodies against flaviviruses.
Lubow et al. (2023)
2 more