› 8dgo

X-ray diffraction
2.3Å resolution

Growth Factor Receptor-Bound Protein 2 (Grb2) bound to phosphorylated PEAK3 (pY24) peptide

Released:
DOI: 10.2210/pdb8dgo/pdb
Model geometry
Fit model/data
PDB entry 8dgo coloured by chain, front view.
PDB entry 8dgo coloured by chain, side view.
PDB entry 8dgo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mapping of PEAK pseudokinase interactions identifies 14-3-3 as a molecular switch for PEAK3 signaling.
Roy MJ, Surudoi MG, Kropp A, Hou J, Dai W, Hardy JM, Liang LY, Cotton TR, Lechtenberg BC, Dite TA, Ma X, Daly RJ, Patel O, Lucet IS
OpenAccess logo Nat Commun 14 3542 (2023)
PMID: 37336884

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274109 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Growth factor receptor-bound protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62993 (Residues: 1-217; Coverage: 100%)
Gene names: ASH, GRB2
Sequence domains:
Protein PEAK3 Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 904 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q6ZS72 (Residues: 23-29; Coverage: 2%)
Gene names: C19orf35, PEAK3

Ligands and Environments

No bound ligands
2 modified residues:
Ligand THC 1 x THC
Ligand PTR 1 x PTR

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43
Unit cell:
a: 89.31Å b: 89.31Å c: 94.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.224
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Location, location, location: Protein kinase nanoclustering for optimised signalling output.
Gormal et al. (2024)
1 other article

1 mention without citation

Structural mapping of PEAK pseudokinase interactions identifies 14-3-3 as a molecular switch for PEAK3 signaling.
Roy et al. (2023)