› 8dhb

Electron Microscopy
3.53Å resolution

Active FLCN GAP complex

Released:
DOI: 10.2210/pdb8dhb/pdb
PDB entry 8dhb coloured by chain, front view.
PDB entry 8dhb coloured by chain, side view.
PDB entry 8dhb coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for FLCN RagC GAP activation in MiT-TFE substrate-selective mTORC1 regulation.
Jansen RM, Peruzzo R, Fromm SA, Yokom AL, Zoncu R, Hurley JH
OpenAccess logo Sci Adv 8 eadd2926 (2022)
PMID: 36103527
Related structures: EMD-27435

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275950 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Ras-related GTP-binding protein C Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 44.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9HB90 (Residues: 1-399; Coverage: 100%)
Gene name: RRAGC
Sequence domains: Gtr1/RagA G protein conserved region
Ras-related GTP-binding protein A Chain: B
Molecule details ›
Chain: B
Length: 336 amino acids
Theoretical weight: 39.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q7L523 (Residues: 1-313; Coverage: 100%)
Gene name: RRAGA
Sequence domains: Gtr1/RagA G protein conserved region
Ragulator complex protein LAMTOR1 Chain: C
Molecule details ›
Chain: C
Length: 166 amino acids
Theoretical weight: 18.33 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6IAA8 (Residues: 1-161; Coverage: 100%)
Gene names: C11orf59, LAMTOR1, PDRO, PP7157
Sequence domains: Late endosomal/lysosomal adaptor and MAPK and MTOR activator
Ragulator complex protein LAMTOR2 Chain: D
Molecule details ›
Chain: D
Length: 127 amino acids
Theoretical weight: 13.65 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y2Q5 (Residues: 1-125; Coverage: 100%)
Gene names: HSPC003, LAMTOR2, MAPBPIP, ROBLD3
Sequence domains: Roadblock/LC7 domain
Ragulator complex protein LAMTOR3 Chain: E
Molecule details ›
Chain: E
Length: 124 amino acids
Theoretical weight: 13.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UHA4 (Residues: 1-124; Coverage: 100%)
Gene names: LAMTOR3, MAP2K1IP1, MAPKSP1, PRO2783
Sequence domains: Mitogen-activated protein kinase kinase 1 interacting
Ragulator complex protein LAMTOR4 Chain: F
Molecule details ›
Chain: F
Length: 99 amino acids
Theoretical weight: 10.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q0VGL1 (Residues: 1-99; Coverage: 100%)
Gene names: C7orf59, LAMTOR4
Ragulator complex protein LAMTOR5 Chain: G
Molecule details ›
Chain: G
Length: 173 amino acids
Theoretical weight: 18.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O43504 (Residues: 1-91; Coverage: 100%)
Gene names: HBXIP, LAMTOR5, XIP
Sequence domains: Ragulator complex protein LAMTOR5
Glutathione S-transferase class-mu 26 kDa isozyme; Neutral amino acid transporter 9 Chain: H
Molecule details ›
Chain: H
Length: 356 amino acids
Theoretical weight: 41 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P08515 (Residues: 2-218; Coverage: 100%)
  • Canonical: Q8NBW4 (Residues: 1-119; Coverage: 21%)
Gene names: SLC38A9, URLC11
Sequence domains:
Folliculin-interacting protein 2 Chain: I
Molecule details ›
Chain: I
Length: 1351 amino acids
Theoretical weight: 149.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9P278 (Residues: 1-1114; Coverage: 100%)
Gene names: FNIP2, FNIPL, KIAA1450, MAPO1
Sequence domains:
Folliculin Chain: J
Molecule details ›
Chain: J
Length: 622 amino acids
Theoretical weight: 69.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NFG4 (Residues: 1-579; Coverage: 100%)
Gene names: BHD, FLCN
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CZC 1 x CZC
Ligand BEF 1 x BEF
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.53Å
Relevant EMDB volumes: EMD-27435
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

5 review citations

Targeting the biology of aging with mTOR inhibitors.
Mannick et al. (2023)
4 more

2 mentions without citation

Longin domain GAP complexes in nutrient signalling, membrane traffic and neurodegeneration.
Jansen et al. (2023)
1 more