› 8dj7

X-ray diffraction
2.39Å resolution

The complex structure between human IgG1 Fc and its high affinity receptor FcgRI H174R variant

Released:
DOI: 10.2210/pdb8dj7/pdb
PDB entry 8dj7 coloured by chain, front view.
PDB entry 8dj7 coloured by chain, side view.
PDB entry 8dj7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
FcγRI FG-loop functions as a pH sensitive switch for IgG binding and release.
Lu J, Spencer M, Zou Z, Traver M, Brzostowski J, Sun PD
OpenAccess logo Front Immunol 14 1100499 (2023)
PMID: 36814926

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-525484 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.7 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 112-328; Coverage: 54%)
  • Best match: P01857-1 (Residues: 112-330)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
High affinity immunoglobulin gamma Fc receptor I Chain: C
Molecule details ›
Chain: C
Length: 277 amino acids
Theoretical weight: 31.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12314 (Residues: 21-289; Coverage: 75%)
Gene names: FCG1, FCGR1, FCGR1A, IGFR1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC, GAL
Carbohydrate polymer
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 206.371Å b: 89.037Å c: 56.191Å
α: 90° β: 96.81° γ: 90°
R-values:
R R work R free
0.217 0.214 0.266
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Broadening anticancer spectrum by preprocessing and treatment of T- lymphocytes expressed FcγRI and monoclonal antibodies for refractory cancers.
Tang et al. (2024)

1 mention without citation

FcγRI FG-loop functions as a pH sensitive switch for IgG binding and release.
Lu et al. (2023)