› 8dke

Electron Microscopy
3.18Å resolution

Cryo-EM structure of cystinosin in a cytosol-open state

Released:
DOI: 10.2210/pdb8dke/pdb
PDB entry 8dke coloured by chain, front view.
PDB entry 8dke coloured by chain, side view.
PDB entry 8dke coloured by chain, top view.
Source organisms:
Primary publication:
Structure and mechanism of human cystine exporter cystinosin.
Guo X, Schmiege P, Assafa TE, Wang R, Xu Y, Donnelly L, Fine M, Ni X, Jiang J, Millhauser G, Feng L, Li X
Cell (2022)
PMID: 36113465
Related structures: EMD-27488

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271799 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cystinosin Chain: P
Molecule details ›
Chain: P
Length: 408 amino acids
Theoretical weight: 46.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O60931 (Residues: 1-362; Coverage: 99%)
  • Best match: O60931-2 (Residues: 1-400)
Gene name: CTNS
Sequence domains: PQ loop repeat
Fab 3H5 Heavy chain Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 26.77 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Fab 3H5 Kappa chain Chain: B
Molecule details ›
Chain: B
Length: 233 amino acids
Theoretical weight: 25.47 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.18Å
Relevant EMDB volumes: EMD-27488
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Advances in Drug Discovery Targeting Lysosomal Membrane Proteins.
Wang et al. (2023)
7 other articles

2 mentions without citation

Structure and mechanism of human cystine exporter cystinosin.
Guo et al. (2022)
1 more