› 8dnh

Electron Microscopy
2.99Å resolution

Cryo-EM structure of nonmuscle beta-actin

Released:
DOI: 10.2210/pdb8dnh/pdb
PDB entry 8dnh coloured by chain, front view.
PDB entry 8dnh coloured by chain, side view.
PDB entry 8dnh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into actin isoforms.
Arora AS, Huang HL, Singh R, Narui Y, Suchenko A, Hatano T, Heissler SM, Balasubramanian MK, Chinthalapudi K
OpenAccess logo Elife 12 (2023)
PMID: 36790143
Related structures: EMD-27572

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, cytoplasmic 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 375 amino acids
Theoretical weight: 41.69 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P60709 (Residues: 2-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ADP 4 x ADP
1 modified residue:
Ligand HIC 4 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 2.99Å
Relevant EMDB volumes: EMD-27572
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Nucleus Mechanosensing in Cardiomyocytes.
Coscarella et al. (2023)
2 other articles

2 mentions without citation

Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function.
Nietmann et al. (2023)
1 more