8do6

Electron Microscopy
3.1Å resolution

The structure of S. epidermidis Cas10-Csm bound to target RNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-235547 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
CRISPR system Cms endoribonuclease Csm3 Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 214 amino acids
Theoretical weight: 24.03 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Staphylococcus epidermidis RP62A
UniProt:
  • Canonical: Q5HK91 (Residues: 1-214; Coverage: 100%)
Gene name: SERP2459
Sequence domains: RAMP superfamily
CRISPR system Cms protein Csm2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 141 amino acids
Theoretical weight: 16.81 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Staphylococcus epidermidis RP62A
UniProt:
  • Canonical: A0A2R3SZR9 (Residues: 1-141; Coverage: 100%)
Sequence domains: Csm2 Type III-A
CRISPR system Cms protein Csm4 Chain: B
CRISPR system Cms protein Csm5 Chain: H
crRNA Chain: I
Target RNA Chain: J

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-27593
Expression system: Staphylococcus epidermidis RP62A