› 8do6

Electron Microscopy
3.1Å resolution

The structure of S. epidermidis Cas10-Csm bound to target RNA

Released:
DOI: 10.2210/pdb8do6/pdb
PDB entry 8do6 coloured by chain, front view.
PDB entry 8do6 coloured by chain, side view.
PDB entry 8do6 coloured by chain, top view.
Source organism: Staphylococcus epidermidis RP62A
Primary publication:
The structure of a Type III-A CRISPR-Cas effector complex reveals conserved and idiosyncratic contacts to target RNA and crRNA among Type III-A systems.
Paraan M, Nasef M, Chou-Zheng L, Khweis SA, Schoeffler AJ, Hatoum-Aslan A, Stagg SM, Dunkle JA
OpenAccess logo PLoS One 18 e0287461 (2023)
PMID: 37352230
Related structures: EMD-27593

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-496878 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
CRISPR system Cms endoribonuclease Csm3 Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 214 amino acids
Theoretical weight: 24.03 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Staphylococcus epidermidis RP62A
UniProt:
  • Canonical: Q5HK91 (Residues: 1-214; Coverage: 100%)
Gene name: SERP2459
Sequence domains: RAMP superfamily
CRISPR system Cms protein Csm2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 141 amino acids
Theoretical weight: 16.81 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Staphylococcus epidermidis RP62A
UniProt:
  • Canonical: A0A2R3SZR9 (Residues: 1-141; Coverage: 100%)
Sequence domains: Csm2 Type III-A
CRISPR system Cms protein Csm4 Chain: B
Molecule details ›
Chain: B
Length: 304 amino acids
Theoretical weight: 34.55 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Staphylococcus epidermidis RP62A
UniProt:
  • Canonical: Q5HK92 (Residues: 1-304; Coverage: 100%)
Gene name: SERP2458
Sequence domains:
CRISPR system Cms protein Csm5 Chain: H
Molecule details ›
Chain: H
Length: 340 amino acids
Theoretical weight: 39.45 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Staphylococcus epidermidis RP62A
UniProt:
  • Canonical: Q5HK93 (Residues: 1-340; Coverage: 100%)
Gene name: SERP2457
Sequence domains: RAMP superfamily
crRNA Chain: I
Molecule details ›
Chain: I
Length: 37 nucleotides
Theoretical weight: 11.9 KDa
Target RNA Chain: J
Molecule details ›
Chain: J
Length: 43 nucleotides
Theoretical weight: 13.6 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-27593
Expression system: Staphylococcus epidermidis RP62A

Quick links

Citations

1 mention without citation

The structure of a Type III-A CRISPR-Cas effector complex reveals conserved and idiosyncratic contacts to target RNA and crRNA among Type III-A systems.
Paraan et al. (2023)