› 8dpf

Electron Microscopy
2.84Å resolution

Cryo-EM structure of the 5HT2C receptor (INI isoform) bound to lorcaserin

Released:
DOI: 10.2210/pdb8dpf/pdb
PDB entry 8dpf coloured by chain, front view.
PDB entry 8dpf coloured by chain, side view.
PDB entry 8dpf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular insights into the regulation of constitutive activity by RNA editing of 5HT2C serotonin receptors.
Gumpper RH, Fay JF, Roth BL
OpenAccess logo Cell Rep 40 111211 (2022)
PMID: 35977511
Related structures: EMD-27633

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-273481 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
5-hydroxytryptamine receptor 2C Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 51.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
G-alpha subunit q (Gi2-mini-Gq chimeric) Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Antibody fragment scFv16 Chain: E
Molecule details ›
Chain: E
Length: 267 amino acids
Theoretical weight: 28.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

2 bound ligands:
Ligand T4U 1 x T4U
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-27633
Expression system: Spodoptera frugiperda

Quick links

Citations

6 review citations

Psychedelics: preclinical insights provide directions for future research.
Gumpper et al. (2024)
5 more

7 mentions without citation

A Novel "Activation Switch" Motif Common to All Aminergic Receptors.
Lee et al. (2023)
6 more