› 8dpm

Electron Microscopy
3Å resolution

Structure of EBOV GP lacking the mucin-like domain with 9.20.1A2 Fab and 6D6 scFv bound

Released:
DOI: 10.2210/pdb8dpm/pdb
PDB entry 8dpm coloured by chain, front view.
PDB entry 8dpm coloured by chain, side view.
PDB entry 8dpm coloured by chain, top view.
Source organisms:
Primary publication:
The evolution and determinants of neutralization of potent head-binding antibodies against Ebola virus.
Yu X, Hastie KM, Davis CW, Avalos RD, Williams D, Parekh D, Hui S, Mann C, Hariharan C, Takada A, Ahmed R, Saphire EO
Cell Rep 42 113366 (2023)
PMID: 37938974
Related structures: EMD-27638

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-554700 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
GP1 Chains: A, F, K
Molecule details ›
Chains: A, F, K
Length: 280 amino acids
Theoretical weight: 31.3 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Drosophila melanogaster
GP2 Chains: B, G, L
Molecule details ›
Chains: B, G, L
Length: 136 amino acids
Theoretical weight: 15.34 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Drosophila melanogaster
Antibody 6D6 scFv Chains: C, H, M
Molecule details ›
Chains: C, H, M
Length: 243 amino acids
Theoretical weight: 25.85 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
Antibody 9.20.1A2 Fab heavy chain Chains: D, I, N
Molecule details ›
Chains: D, I, N
Length: 122 amino acids
Theoretical weight: 13.34 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Antibody 9.20.1A2 Fab light chain Chains: E, J, O
Molecule details ›
Chains: E, J, O
Length: 112 amino acids
Theoretical weight: 11.99 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-27638
Expression systems:
  • Drosophila melanogaster
  • Cricetulus griseus

Quick links

Citations

2 citation in other articles

Computational mining of B cell receptor repertoires reveals antigen-specific and convergent responses to Ebola vaccination.
Richardson et al. (2024)
1 more

2 mentions without citation

Local adaptation at higher trophic levels: contrasting hyperparasite-pathogen infection dynamics in the field and laboratory.
Parratt et al. (2017)
1 more