› 8dss

X-ray diffraction
3.05Å resolution

X-ray crystal structure of Geobacillus stearothermophilus ComEA

Released:
DOI: 10.2210/pdb8dss/pdb
PDB entry 8dss coloured by chain, front view.
PDB entry 8dss coloured by chain, side view.
PDB entry 8dss coloured by chain, top view.
Source organism: Geobacillus stearothermophilus ATCC 7953
Primary publication:
Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria.
Ahmed I, Hahn J, Henrickson A, Khaja FT, Demeler B, Dubnau D, Neiditch MB
OpenAccess logo Nat Commun 13 7724 (2022)
PMID: 36513643

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helix-hairpin-helix DNA-binding motif class 1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 15.66 KDa
Source organism: Geobacillus stearothermophilus ATCC 7953
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K9HI54 (Residues: 60-207; Coverage: 72%)
Gene names: B4109_2104, B4114_2221, D9548_03605
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P65
Unit cell:
a: 80.706Å b: 80.706Å c: 71.279Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.226 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

A spectral decomposition quality assessment tool for multi-wavelength AUC experiments with UltraScan.
Mortezazadeh et al. (2023)
3 more

4 mentions without citation

Engineered Biomaterials Trigger Remineralization and Antimicrobial Effects for Dental Caries Restoration.
Li et al. (2023)
3 more