› 8dv4

X-ray diffraction
2.4Å resolution

Crystal structure of the BC8B TCR-CD1b-PI complex

Released:
DOI: 10.2210/pdb8dv4/pdb
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PDB entry 8dv4 coloured by chain, front view.
PDB entry 8dv4 coloured by chain, side view.
PDB entry 8dv4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
αβ T-cell receptor recognition of self-phosphatidylinositol presented by CD1b.
Farquhar R, Van Rhijn I, Moody DB, Rossjohn J, Shahine A
OpenAccess logo J Biol Chem 299 102849 (2023)
PMID: 36587766

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151323 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
T-cell surface glycoprotein CD1b Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.53 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P29016 (Residues: 20-296; Coverage: 88%)
Gene name: CD1B
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
T-cell receptor alpha variable TRAV9-2 Chain: D
T-cell receptor beta variable TRBV6-2 Chain: E

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Ligand MAN 1 x MAN
Ligand NAG 2 x NAG
Ligand BMA 1 x BMA
Carbohydrate polymer : NEW Components: NAG, BMA
Ligand NAG 2 x NAG
Ligand BMA 1 x BMA
8 bound ligands:
Ligand EDO 6 x EDO
Ligand GOL 2 x GOL
Ligand MAN 1 x MAN
Ligand NAG 1 x NAG
Ligand CUY 1 x CUY
Ligand CL 3 x CL
Ligand NA 3 x NA
Ligand PIE 1 x PIE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 73.088Å b: 65.43Å c: 101.581Å
α: 90° β: 102.26° γ: 90°
R-values:
R R work R free
0.195 0.192 0.254
Expression systems:
  • Homo sapiens
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 review citation

TCR-T cell therapy: current development approaches, preclinical evaluation, and perspectives on regulatory challenges.
Golikova et al. (2024)

1 mention without citation

αβ T-cell receptor recognition of self-phosphatidylinositol presented by CD1b.
Farquhar et al. (2023)

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