› 8dzm

X-ray diffraction
1.65Å resolution

Crystal structure of human Sar1a in complex with ppGpp and Magnesium

Released:
DOI: 10.2210/pdb8dzm/pdb
PDB entry 8dzm coloured by chain, front view.
PDB entry 8dzm coloured by chain, side view.
PDB entry 8dzm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The alarmone ppGpp selectively inhibits the isoform A of the human small GTPase Sar1.
Huang Q, Szebenyi DME
Proteins (2022)
PMID: 36369712

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small COPII coat GTPase SAR1A Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR31 (Residues: 1-198; Coverage: 100%)
Gene names: SAR1, SAR1A, SARA, SARA1
Sequence domains: ADP-ribosylation factor family

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand G4P 2 x G4P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 136.125Å b: 35.933Å c: 76.118Å
α: 90° β: 101.52° γ: 90°
R-values:
R R work R free
0.171 0.17 0.197
Expression system: Escherichia coli

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