› 8dzq

Electron Microscopy
2.82Å resolution

momSalB bound Kappa Opioid Receptor in complex with GoA

Released:
DOI: 10.2210/pdb8dzq/pdb
PDB entry 8dzq coloured by chain, front view.
PDB entry 8dzq coloured by chain, side view.
PDB entry 8dzq coloured by chain, top view.
Source organisms:
Primary publication:
Ligand and G-protein selectivity in the κ-opioid receptor.
Han J, Zhang J, Nazarova AL, Bernhard SM, Krumm BE, Zhao L, Lam JH, Rangari VA, Majumdar S, Nichols DE, Katritch V, Yuan P, Fay JF, Che T
Nature (2023)
PMID: 37138078
Related structures: EMD-27805

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225117 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
ScFv16 protein Chain: E
Molecule details ›
Chain: E
Length: 251 amino acids
Theoretical weight: 26.68 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(o) subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 40.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P09471 (Residues: 1-354; Coverage: 100%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Kappa-type opioid receptor Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 34.93 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41145 (Residues: 54-358; Coverage: 80%)
Gene names: OPRK, OPRK1
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand U99 1 x U99
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.82Å
Relevant EMDB volumes: EMD-27805
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Involvement of the Opioid Peptide Family in Cancer Progression.
Sánchez et al. (2023)
1 more

1 mention without citation

IUPHAR themed review: Opioid efficacy, bias, and selectivity.
Ramos-Gonzalez et al. (2023)