› 8dzt

X-ray diffraction
1.8Å resolution

Crystal structure of human Sar1aH79G mutant

Released:
DOI: 10.2210/pdb8dzt/pdb
Model geometry
Fit model/data
PDB entry 8dzt coloured by chain, front view.
PDB entry 8dzt coloured by chain, side view.
PDB entry 8dzt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The alarmone ppGpp selectively inhibits the isoform A of the human small GTPase Sar1.
Huang Q, Szebenyi DME
Proteins (2022)
PMID: 36369712

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small COPII coat GTPase SAR1A Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR31 (Residues: 1-198; Coverage: 100%)
Gene names: SAR1, SAR1A, SARA, SARA1
Sequence domains: ADP-ribosylation factor family

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand CA 4 x CA
Ligand G4P 2 x G4P
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CHESS BEAMLINE A1
Spacegroup: P61
Unit cell:
a: 55.718Å b: 55.718Å c: 222.972Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.152 0.188
Expression system: Escherichia coli

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