› 8e10

X-ray diffraction
1.65Å resolution

Structure of mouse polymerase beta

Released:
DOI: 10.2210/pdb8e10/pdb
PDB entry 8e10 coloured by chain, front view.
PDB entry 8e10 coloured by chain, side view.
PDB entry 8e10 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Polβ/XRCC1 heterodimerization dictates DNA damage recognition and basal Polβ protein levels without interfering with mouse viability or fertility.
Koczor CA, Thompson MK, Sharma N, Prakash A, Sobol RW
DNA Repair (Amst) 123 103452 (2023)
PMID: 36702010

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-569122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8K409 (Residues: 88-335; Coverage: 74%)
Gene name: Polb
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MLA 2 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER IMUS 3.0 MICROFOCUS
Spacegroup: P21212
Unit cell:
a: 62.725Å b: 120.534Å c: 69.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.173 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

4 mentions without citation

Potent neutralization of botulinum neurotoxin/B by synergistic action of antibodies recognizing protein and ganglioside receptor binding domain.
Chen et al. (2012)
3 more