8e30

Electron Microscopy
1.91Å resolution

E. coli 50S ribosome bound to compound streptogramin A analog 3142

Released:
Source organism: Escherichia coli
Entry authors: Pellegrino J, Lee DJ, Fraser JS, Seiple IB
Related structures: EMD-27852

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-232402 (preferred)
Entry contents:
8 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (10 distinct):
Large ribosomal subunit protein uL2 Chain: K
Molecule details ›
Chain: K
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 2-272; Coverage: 99%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL15 Chain: L
Molecule details ›
Chain: L
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02413 (Residues: 1-144; Coverage: 100%)
Gene names: JW3263, b3301, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL4 Chain: M
Molecule details ›
Chain: M
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Gene names: JW3281, b3319, eryA, rplD
Sequence domains: Ribosomal protein L4/L1 family
Large ribosomal subunit protein uL3 Chain: N
Molecule details ›
Chain: N
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL13 Chain: O
Molecule details ›
Chain: O
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA10 (Residues: 1-142; Coverage: 100%)
Gene names: JW3200, b3231, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL22 Chain: P
Molecule details ›
Chain: P
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P61175 (Residues: 1-110; Coverage: 100%)
Gene names: JW3277, b3315, eryB, rplV
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein bL32 Chain: Q
Molecule details ›
Chain: Q
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7N4 (Residues: 2-57; Coverage: 98%)
Gene names: JW1075, b1089, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL34 Chain: R
Molecule details ›
Chain: R
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Sequence domains: Ribosomal protein L34
23S ribosomal RNA Chain: I
Molecule details ›
Chain: I
Length: 2904 nucleotides
Theoretical weight: 941.8 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
5S ribosomal RNA Chain: J
Molecule details ›
Chain: J
Length: 118 nucleotides
Theoretical weight: 38.18 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 1.91Å
Relevant EMDB volumes: EMD-27852
Expression system: Escherichia coli