› 8e3e

X-ray diffraction
2.99Å resolution

ZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing CAST sequence (#10)

Released:
DOI: 10.2210/pdb8e3e/pdb
PDB entry 8e3e coloured by chain, front view.
PDB entry 8e3e coloured by chain, side view.
PDB entry 8e3e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for transcription factor ZBTB7A recognition of DNA and effects of ZBTB7A somatic mutations that occur in human acute myeloid leukemia.
Ren R, Horton JR, Chen Q, Yang J, Liu B, Huang Y, Blumenthal RM, Zhang X, Cheng X
OpenAccess logo J Biol Chem 102885 (2023)
PMID: 36626981

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-512629 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Zinc finger and BTB domain-containing protein 7A Chains: A, B, F
Molecule details ›
Chains: A, B, F
Length: 165 amino acids
Theoretical weight: 19.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95365 (Residues: 341-505; Coverage: 28%)
Gene names: FBI1, LRF, ZBTB7, ZBTB7A, ZNF857A
Sequence domains: Zinc finger, C2H2 type
DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3') Chains: D, H, X
Molecule details ›
Chains: D, H, X
Length: 21 nucleotides
Theoretical weight: 6.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3') Chains: E, I, Y
Molecule details ›
Chains: E, I, Y
Length: 21 nucleotides
Theoretical weight: 6.37 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 67.519Å b: 147.175Å c: 68.064Å
α: 90° β: 96.46° γ: 90°
R-values:
R R work R free
0.205 0.203 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 citations in other articles

Modulation of neural gene networks by estradiol in old rhesus macaque females.
Cervera-Juanes et al. (2024)

2 mentions without citation

Anthocyanin-Rich Vegetables for Human Consumption-Focus on Potato, Sweetpotato and Tomato.
Mattoo et al. (2022)
1 more