› 8e3i

Electron Microscopy
2.53Å resolution

CRYO-EM STRUCTURE OF the human MPSF IN COMPLEX WITH THE AUUAAA poly(A) signal

Released:
DOI: 10.2210/pdb8e3i/pdb
PDB entry 8e3i coloured by chain, front view.
PDB entry 8e3i coloured by chain, side view.
PDB entry 8e3i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the recognition of the AUUAAA polyadenylation signal by mPSF.
Gutierrez PA, Wei J, Sun Y, Tong L
OpenAccess logo RNA 28 1534-1541 (2022)
PMID: 36130077
Related structures: EMD-27866

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131974 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Cleavage and polyadenylation specificity factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1443 amino acids
Theoretical weight: 161.07 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q10570 (Residues: 1-1443; Coverage: 100%)
Gene names: CPSF1, CPSF160
Sequence domains:
Cleavage and polyadenylation specificity factor subunit 4 Chain: C
Molecule details ›
Chain: C
Length: 245 amino acids
Theoretical weight: 27.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95639 (Residues: 1-269; Coverage: 91%)
  • Best match: O95639-2 (Residues: 1-244)
Gene names: CPSF30, CPSF4, NAR, NEB1
Sequence domains:
pre-mRNA 3' end processing protein WDR33 Chain: B
Molecule details ›
Chain: B
Length: 572 amino acids
Theoretical weight: 65.91 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9C0J8 (Residues: 1-572; Coverage: 43%)
Gene names: WDC146, WDR33
Sequence domains: WD domain, G-beta repeat
RNA (5'-R(P*CP*AP*UP*UP*AP*AP*AP*CP*AP*AP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 11 nucleotides
Theoretical weight: 3.46 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.53Å
Relevant EMDB volumes: EMD-27866
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Coevolution combined with molecular dynamics simulations provides structural and mechanistic insights into the interactions between the integrator complex subunits.
Fongang et al. (2023)