› 8e3x

Electron Microscopy
2.3Å resolution

Cryo-EM structure of the PAC1R-PACAP27-Gs complex

Released:
DOI: 10.2210/pdb8e3x/pdb
PDB entry 8e3x coloured by chain, front view.
PDB entry 8e3x coloured by chain, side view.
PDB entry 8e3x coloured by chain, top view.
Source organisms:
Primary publication:
Understanding VPAC receptor family peptide binding and selectivity.
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
OpenAccess logo Nat Commun 13 7013 (2022)
PMID: 36385145
Related structures: EMD-27872

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-273289 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Pituitary adenylate cyclase-activating polypeptide 27 Chain: P
Molecule details ›
Chain: P
Length: 27 amino acids
Theoretical weight: 3.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P18509 (Residues: 132-158; Coverage: 18%)
Gene name: ADCYAP1
Sequence domains: Peptide hormone
Pituitary adenylate cyclase-activating polypeptide type I receptor Chain: R
Molecule details ›
Chain: R
Length: 483 amino acids
Theoretical weight: 55.44 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P41586 (Residues: 19-468; Coverage: 100%)
Gene name: ADCYAP1R1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.3Å
Relevant EMDB volumes: EMD-27872
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

Differential Responses of the GLP-1 and GLP-2 Receptors to N-Terminal Modification of a Dual Agonist.
Gibadullin et al. (2023)
1 more