› 8e52

Electron crystallography
2.8Å resolution

MicroED structure of proteinase K recorded on K2

Released:
DOI: 10.2210/pdb8e52/pdb
PDB entry 8e52 coloured by chain, front view.
PDB entry 8e52 coloured by chain, side view.
PDB entry 8e52 coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Electron-counting MicroED data with the K2 and K3 direct electron detectors.
Clabbers MTB, Martynowycz MW, Hattne J, Nannenga BL, Gonen T
OpenAccess logo J Struct Biol 107886 (2022)
PMID: 36044956
Related structures: EMD-27900

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: AAA
Molecule details ›
Chain: AAA
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
Expression system: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-27900
Expression system: Parengyodontium album

Quick links

Citations

2 citation in other articles

Electron counting with direct electron detectors in MicroED.
Hattne et al. (2023)
1 more