› 8e71

X-ray diffraction
1.57Å resolution

Staphylococcus aureus ClpP in complex with compound 3471

Released:
DOI: 10.2210/pdb8e71/pdb
PDB entry 8e71 coloured by chain, front view.
PDB entry 8e71 coloured by chain, side view.
PDB entry 8e71 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Entry authors: Lee RE, Griffith EC

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent Clp protease proteolytic subunit Chains: A, B, C, D, E, F, G, I, K, L, M, N, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, I, K, L, M, N, S, T
Length: 203 amino acids
Theoretical weight: 22.61 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2G036 (Residues: 1-195; Coverage: 100%)
Gene names: SAOUHSC_00790, clpP
Sequence domains: Clp protease

Ligands and Environments

2 bound ligands:
Ligand MPD 16 x MPD
Ligand USU 14 x USU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 94.68Å b: 126.994Å c: 145.851Å
α: 90° β: 93.97° γ: 90°
R-values:
R R work R free
0.156 0.156 0.171
Expression system: Escherichia coli BL21(DE3)

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