› 8e74

Electron Microscopy
2.94Å resolution

Mycobacterium tuberculosis RNAP paused elongation complex with NusG transcription factor

Released:
DOI: 10.2210/pdb8e74/pdb
PDB entry 8e74 coloured by chain, front view.
PDB entry 8e74 coloured by chain, side view.
PDB entry 8e74 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural and functional basis of the universal transcription factor NusG pro-pausing activity in Mycobacterium tuberculosis.
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell 83 1474-1488.e8 (2023)
PMID: 37116494
Related structures: EMD-27935

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-229700 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
Transcription termination/antitermination protein NusG Chain: Z
Molecule details ›
Chain: Z
Length: 238 amino acids
Theoretical weight: 25.44 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIU9 (Residues: 2-238; Coverage: 100%)
Gene names: MTCY20H10.20, Rv0639, nusG
Sequence domains: Transcription termination factor nusG
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 37.75 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGZ1 (Residues: 1-347; Coverage: 100%)
Gene names: MTCY13E12.10c, Rv3457c, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1172 amino acids
Theoretical weight: 129.37 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY9 (Residues: 7-1178; Coverage: 100%)
Gene names: MTCI376.08c, Rv0667, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1318 amino acids
Theoretical weight: 147.1 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY7 (Residues: 1-1316; Coverage: 100%)
Gene names: MTCI376.07c, Rv0668, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 110 amino acids
Theoretical weight: 11.82 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY5 (Residues: 1-110; Coverage: 100%)
Gene names: MTCY21B4.07, Rv1390, rpoZ
Sequence domains: RNA polymerase Rpb6
DNA (54-MER) Chain: O
Molecule details ›
Chain: O
Length: 54 nucleotides
Theoretical weight: 16.55 KDa
DNA (54-MER) Chain: P
Molecule details ›
Chain: P
Length: 54 nucleotides
Theoretical weight: 16.71 KDa
RNA (42-MER) Chain: R
Molecule details ›
Chain: R
Length: 42 nucleotides
Theoretical weight: 13.36 KDa

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.94Å
Relevant EMDB volumes: EMD-27935
Expression system: Escherichia coli

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