› 8e8u

X-ray diffraction
2.65Å resolution

Structure of the LOR domain of human AASS

Released:
DOI: 10.2210/pdb8e8u/pdb
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PDB entry 8e8u coloured by chain, front view.
PDB entry 8e8u coloured by chain, side view.
PDB entry 8e8u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterization and structure of the human lysine-2-oxoglutarate reductase domain, a novel therapeutic target for treatment of glutaric aciduria type 1.
Leandro J, Khamrui S, Suebsuwong C, Chen PJ, Secor C, Dodatko T, Yu C, Sanchez R, DeVita RJ, Houten SM, Lazarus MB
OpenAccess logo Open Biol 12 220179 (2022)
PMID: 36128717

Function and Biology Details

Reactions catalysed: 
N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP(+) + H(2)O = L-lysine + 2-oxoglutarate + NADPH
N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD(+) + H(2)O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-aminoadipic semialdehyde synthase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 452 amino acids
Theoretical weight: 50.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UDR5 (Residues: 21-470; Coverage: 49%)
Gene name: AASS
Sequence domains: Alanine dehydrogenase/PNT, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P21
Unit cell:
a: 73.005Å b: 131.944Å c: 96.494Å
α: 90° β: 100.72° γ: 90°
R-values:
R R work R free
0.28 0.278 0.319
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

MultifacetedProtDB: a database of human proteins with multiple functions.
Bertolini et al. (2024)

2 mentions without citation

Characterization and structure of the human lysine-2-oxoglutarate reductase domain, a novel therapeutic target for treatment of glutaric aciduria type 1.
Leandro et al. (2022)
1 more

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