› › Structure analysis

Cryo-EM structure of substrate-free ClpX.ClpP

Electron Microscopy
2.57Å resolution
PDB entry 8e91 coloured by chain, front view.
PDB entry 8e91 coloured by chain, side view.
PDB entry 8e91 coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
hetero icosamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Endopeptidase ClpP complex and ATP-dependent Clp protease ATP-binding subunit ClpX
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Multimeric state: hetero icosamer
Accessible surface area: 172497.67 Å2
Buried surface area: 100588.77 Å2
Dissociation area: 5,163.8 Å2
Dissociation energy (ΔGdiss): 65.65 kcal/mol
Dissociation entropy (TΔSdiss): 17.33 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-141328

Macromolecules

ATP-dependent Clp protease ATP-binding subunit ClpX
Chains: A, B, C, D, E, F
Length: 424 amino acids
Theoretical weight: 46.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6H1 (Residues: 1-424; Coverage: 100%)
Gene names: JW0428, b0438, clpX, lopC
Pfam:
ATP-dependent Clp protease ATP-binding subunit ClpX in PDB entry 8e91, assembly 1, front view.
ATP-dependent Clp protease ATP-binding subunit ClpX in PDB entry 8e91, assembly 1, side view.
ATP-dependent Clp protease ATP-binding subunit ClpX in PDB entry 8e91, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P0A6H1  
142450100150200250300350400
 
100200300400MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLCNDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVENIIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKAKSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALTKQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMEDVEKVVIDESVIDGQSEPLLIYGKPEAQQASGE
UniProt
P0A6H1
Chains
Ligand binding sites
Interaction interfaces
Sequence conservation
Molecule details ›

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ATP-dependent Clp protease proteolytic subunit
Chains: H, I, J, K, L, M, N, h, i, j, k, l, m, n
Length: 207 amino acids
Theoretical weight: 23.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6G7 (Residues: 16-207; Coverage: 93%)
Gene names: JW0427, b0437, clpP, lopP
Pfam: Clp protease
ATP-dependent Clp protease proteolytic subunit in PDB entry 8e91, assembly 1, front view.
ATP-dependent Clp protease proteolytic subunit in PDB entry 8e91, assembly 1, side view.
ATP-dependent Clp protease proteolytic subunit in PDB entry 8e91, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P0A6G7  
120720406080100120140160180200
 
50100150200LVPMVIEQTSRGERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVKGRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRNENLYFQSLEHHHHHH
UniProt
P0A6G7
Chains
Interaction interfaces
Sequence conservation
Molecule details ›

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