› 8e9g

Electron Microscopy
2.6Å resolution

Mycobacterial respiratory complex I with both quinone positions modelled

Released:
DOI: 10.2210/pdb8e9g/pdb
PDB entry 8e9g coloured by chain, front view.
PDB entry 8e9g coloured by chain, side view.
PDB entry 8e9g coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structure of mycobacterial respiratory complex I.
Liang Y, Plourde A, Bueler SA, Liu J, Brzezinski P, Vahidi S, Rubinstein JL
OpenAccess logo Proc Natl Acad Sci U S A 120 e2214949120 (2023)
PMID: 36952383
Related structures: EMD-27963

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-106325 (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
Response regulatory domain-containing protein Chain: O
Molecule details ›
Chain: O
Length: 132 amino acids
Theoretical weight: 14.12 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU37 (Residues: 1-132; Coverage: 100%)
Gene name: MSMEG_2064
NADH-quinone oxidoreductase subunit B Chain: B
Molecule details ›
Chain: B
Length: 184 amino acids
Theoretical weight: 20.12 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU35 (Residues: 1-184; Coverage: 100%)
Gene names: MSMEG_2062, MSMEI_2017, nuoB
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
NADH-quinone oxidoreductase subunit A Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 13.63 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU36 (Residues: 1-122; Coverage: 100%)
Gene names: MSMEG_2063, nuoA
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
NADH-quinone oxidoreductase subunit C Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.61 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU34 (Residues: 1-238; Coverage: 100%)
Gene names: MSMEG_2061, MSMEI_2016, nuoC
Sequence domains: Respiratory-chain NADH dehydrogenase, 30 Kd subunit
NADH-quinone oxidoreductase subunit D Chain: D
Molecule details ›
Chain: D
Length: 442 amino acids
Theoretical weight: 48.56 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU33 (Residues: 1-442; Coverage: 100%)
Gene names: MSMEG_2060, MSMEI_2015, nuoD
Sequence domains: Respiratory-chain NADH dehydrogenase, 49 Kd subunit
NADH-quinone oxidoreductase chain e Chain: E
Molecule details ›
Chain: E
Length: 245 amino acids
Theoretical weight: 25.75 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU32 (Residues: 1-245; Coverage: 100%)
Gene name: MSMEG_2059
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-quinone oxidoreductase Chain: G
Molecule details ›
Chain: G
Length: 794 amino acids
Theoretical weight: 83.89 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU30 (Residues: 1-794; Coverage: 100%)
Gene names: MSMEG_2057, nuoG
Sequence domains:
NADH-quinone oxidoreductase subunit F Chain: F
Molecule details ›
Chain: F
Length: 443 amino acids
Theoretical weight: 47.83 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU31 (Residues: 1-443; Coverage: 100%)
Gene names: MSMEG_2058, nuoF
Sequence domains:
NADH-quinone oxidoreductase subunit I Chain: I
Molecule details ›
Chain: I
Length: 180 amino acids
Theoretical weight: 19.86 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU28 (Residues: 1-180; Coverage: 100%)
Gene names: MSMEG_2055, MSMEI_2010, nuoI
Sequence domains: 4Fe-4S dicluster domain
NADH-quinone oxidoreductase subunit H Chain: H
Molecule details ›
Chain: H
Length: 408 amino acids
Theoretical weight: 44.48 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU29 (Residues: 1-408; Coverage: 100%)
Gene names: MSMEG_2056, MSMEI_2011, nuoH
Sequence domains: NADH dehydrogenase
NADH-quinone oxidoreductase subunit J Chain: J
Molecule details ›
Chain: J
Length: 252 amino acids
Theoretical weight: 26.4 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU27 (Residues: 1-252; Coverage: 100%)
Gene name: MSMEG_2054
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
NADH-quinone oxidoreductase subunit K Chain: K
Molecule details ›
Chain: K
Length: 99 amino acids
Theoretical weight: 10.88 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU26 (Residues: 1-99; Coverage: 100%)
Gene names: MSMEG_2053, MSMEI_2008, nuoK
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH-quinone oxidoreductase, L subunit Chain: L
Molecule details ›
Chain: L
Length: 629 amino acids
Theoretical weight: 65.45 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU25 (Residues: 1-629; Coverage: 100%)
Gene names: MSMEG_2052, nuoL
Sequence domains:
NADH-quinone oxidoreductase subunit N Chain: N
Molecule details ›
Chain: N
Length: 521 amino acids
Theoretical weight: 54.01 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU23 (Residues: 1-521; Coverage: 100%)
Gene names: MSMEG_2050, nuoN
Sequence domains: Proton-conducting membrane transporter
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit domain-containing protein Chain: M
Molecule details ›
Chain: M
Length: 529 amino acids
Theoretical weight: 56.34 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QU24 (Residues: 1-529; Coverage: 100%)
Gene names: MSMEG_2051, nuoM
Sequence domains: Proton-conducting membrane transporter

Ligands and Environments

7 bound ligands:
Ligand SF4 7 x SF4
Ligand MQ9 2 x MQ9
Ligand FES 2 x FES
Ligand GTP 1 x GTP
Ligand ZN 1 x ZN
Ligand FMN 1 x FMN
Ligand XP2 1 x XP2
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-27963

Quick links

Citations

3 citation in other articles

Structures of Tetrahymena thermophila respiratory megacomplexes on the tubular mitochondrial cristae.
Han et al. (2023)
2 more