› 8eaf

Electron Microscopy
2.62Å resolution

SsoMCM hexamer bound to Mg/ADP-BeFx and 12-mer oligo-dT. Class 1

Released:
DOI: 10.2210/pdb8eaf/pdb
PDB entry 8eaf coloured by chain, front view.
PDB entry 8eaf coloured by chain, side view.
PDB entry 8eaf coloured by chain, top view.
Source organisms:
Primary publication:
Two Distinct Modes of DNA Binding by an MCM Helicase Enable DNA Translocation.
Meagher M, Myasnikov A, Enemark EJ
OpenAccess logo Int J Mol Sci 23 (2022)
PMID: 36499022
Related structures: EMD-27974

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-512650 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Minichromosome maintenance protein MCM Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 610 amino acids
Theoretical weight: 68.64 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UXG1 (Residues: 2-612; Coverage: 89%)
Gene names: MCM, SSO0774
Sequence domains:
12-mer oligo dT Chain: X
Molecule details ›
Chain: X
Length: 12 nucleotides
Theoretical weight: 3.61 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand MG 5 x MG
Ligand 08T 5 x 08T
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.62Å
Relevant EMDB volumes: EMD-27974
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

The Response of the Replication Apparatus to Leading Template Strand Blocks.
Bellani et al. (2023)