› 8ec5

X-ray diffraction
1.22Å resolution

Structures of HLA-B8E76C loaded with long peptides reveal novel features at the N-terminus of the groove

Released:
DOI: 10.2210/pdb8ec5/pdb
PDB entry 8ec5 coloured by chain, front view.
PDB entry 8ec5 coloured by chain, side view.
PDB entry 8ec5 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of MHC class I complexes reveal the elusive intermediate conformations explored during peptide editing.
Li L, Peng X, Batliwala M, Bouvier M
OpenAccess logo Nat Commun 14 5020 (2023)
PMID: 37596268

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-235850 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SS57 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, MICA
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
peptide RARARARARARAFVKKKYCL Chain: C
Molecule details ›
Chain: C
Length: 20 amino acids
Theoretical weight: 2.4 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 50.758Å b: 81.149Å c: 110.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.212
Expression systems:
  • Escherichia coli
  • Not provided

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