› 8ee1

X-ray diffraction
2.7Å resolution

KS-AT didomain from module 2 of the 6-deoxyerythronolide B synthase in complex with antibody fragment AA5

Released:
DOI: 10.2210/pdb8ee1/pdb
PDB entry 8ee1 coloured by chain, front view.
PDB entry 8ee1 coloured by chain, side view.
PDB entry 8ee1 coloured by chain, top view.
Source organisms:
Primary publication:
Discovery and Characterization of Antibody Probes of Module 2 of the 6-Deoxyerythronolide B Synthase.
Guzman KM, Cogan DP, Brodsky KL, Soohoo AM, Li X, Sevillano N, Mathews II, Nguyen KP, Craik CS, Khosla C
Biochemistry (2023)
PMID: 37184546

Function and Biology Details

Reaction catalysed: 
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274425 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
6-deoxyerythronolide-B synthase EryA1, modules 1 and 2 Chains: A, D
Molecule details ›
Chains: A, D
Length: 932 amino acids
Theoretical weight: 97.6 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03131 (Residues: 1980-2035, 2036-2867; Coverage: 25%)
Gene name: eryA
Sequence domains:
AA5 antibody heavy chain Chains: B, G
Molecule details ›
Chains: B, G
Length: 249 amino acids
Theoretical weight: 26.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
AA5 antibody light chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 231 amino acids
Theoretical weight: 25.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21212
Unit cell:
a: 249.37Å b: 252.44Å c: 63.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.225 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Chalkophomycin Biosynthesis Revealing Unique Enzyme Architecture for a Hybrid Nonribosomal Peptide Synthetase and Polyketide Synthase.
Yang et al. (2024)

1 mention without citation

Discovery and Characterization of Antibody Probes of Module 2 of the 6-Deoxyerythronolide B Synthase.
Guzman et al. (2023)