› 8ef9

Electron Microscopy
2.4Å resolution

Structure of Lates calcarifer DNA polymerase theta polymerase domain with long duplex DNA, complex Ia

Released:
DOI: 10.2210/pdb8ef9/pdb
PDB entry 8ef9 coloured by chain, front view.
PDB entry 8ef9 coloured by chain, side view.
PDB entry 8ef9 coloured by chain, top view.
Source organism: Lates calcarifer
Primary publication:
Structural basis of DNA polymerase θ mediated DNA end joining.
Li C, Zhu H, Jin S, Maksoud LM, Jain N, Sun J, Gao Y
Nucleic Acids Res (2022)
PMID: 36583344
Related structures: EMD-28075

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221576 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase theta Chain: A
Molecule details ›
Chain: A
Length: 864 amino acids
Theoretical weight: 95.67 KDa
Source organism: Lates calcarifer
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A4W6BKE5 (Residues: 1947-2593; Coverage: 25%)
Gene name: POLQ
Sequence domains: DNA polymerase family A
DNA (5'-D(*AP*GP*CP*TP*CP*TP*AP*CP*GP*GP*AP*TP*GP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 29 nucleotides
Theoretical weight: 8.86 KDa
Source organism: Lates calcarifer
Expression system: Not provided
DNA (5'-D(*AP*GP*CP*AP*TP*CP*CP*GP*TP*AP*GP*(2DA))-3') Chain: F
Molecule details ›
Chain: F
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Lates calcarifer
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand DGT 1 x DGT
Ligand MG 1 x MG
1 modified residue:
Ligand 2DA 1 x 2DA

Experiments and Validation Details

Entry percentile scores
Resolution: 2.4Å
Relevant EMDB volumes: EMD-28075
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

1 review citation

Multifaceted Nature of DNA Polymerase θ.
Kruchinin et al. (2023)
1 other article

1 mention without citation

Structural basis of DNA polymerase θ mediated DNA end joining.
Li et al. (2023)