› 8efv

Electron Microscopy
2.97Å resolution

Structure of single homo-hexameric Holliday junction ATP-dependent DNA helicase RuvB motor

Released:
DOI: 10.2210/pdb8efv/pdb
PDB entry 8efv coloured by chain, front view.
PDB entry 8efv coloured by chain, side view.
PDB entry 8efv coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Shen ZF, Rish AD, Fu TM
Related structures: EMD-28101

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215351 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Holliday junction branch migration complex subunit RuvB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 324 amino acids
Theoretical weight: 36.02 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SL87 (Residues: 1-324; Coverage: 100%)
Gene names: TTHA0406, ruvB
Sequence domains:
49-mer DNA Chain: G
Molecule details ›
Chain: G
Length: 49 nucleotides
Theoretical weight: 15.15 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided
51-mer DNA Chain: H
Molecule details ›
Chain: H
Length: 51 nucleotides
Theoretical weight: 15.69 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand AGS 3 x AGS
Ligand MG 4 x MG
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.97Å
Relevant EMDB volumes: EMD-28101
Expression systems:
  • Escherichia coli
  • Not provided

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