› 8eih

Electron Microscopy
3.04Å resolution

Cryo-EM structure of human DNMT3B homo-tetramer (form I)

Released:
DOI: 10.2210/pdb8eih/pdb
PDB entry 8eih coloured by chain, front view.
PDB entry 8eih coloured by chain, side view.
PDB entry 8eih coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the allosteric regulation and dynamic assembly of DNMT3B.
Lu J, Fang J, Zhu H, Liang KL, Khudaverdyan N, Song J
OpenAccess logo Nucleic Acids Res (2023)
PMID: 37941146
Related structures: EMD-28156

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA (cytosine-5)-methyltransferase 3B Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 650 amino acids
Theoretical weight: 73.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBC3 (Residues: 206-853; Coverage: 76%)
Gene name: DNMT3B
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.04Å
Relevant EMDB volumes: EMD-28156
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Intrafamily heterooligomerization as an emerging mechanism of methyltransferase regulation.
Hobble et al. (2024)