› 8eit

Electron Microscopy
2.8Å resolution

Structure of FFAR1-Gq complex bound to DHA

Released:
DOI: 10.2210/pdb8eit/pdb
PDB entry 8eit coloured by chain, front view.
PDB entry 8eit coloured by chain, side view.
PDB entry 8eit coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism of fatty acid activation of FFAR1.
Kumari P, Inoue A, Chapman K, Lian P, Rosenbaum DM
OpenAccess logo Proc Natl Acad Sci U S A 120 e2219569120 (2023)
PMID: 37216523
Related structures: EMD-28164

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-224875 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
A modified Guanine nucleotide-binding protein G(q) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 82 amino acids
Theoretical weight: 9.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 2-71; Coverage: 99%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 318 amino acids
Theoretical weight: 34.93 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Endolysin; Free fatty acid receptor 1 Chain: R
Molecule details ›
Chain: R
Length: 501 amino acids
Theoretical weight: 54.3 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
  • Canonical: O14842 (Residues: 2-300; Coverage: 100%)
Gene names: E, FFAR1, GPR40
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand HXA 1 x HXA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-28164
Expression system: Spodoptera frugiperda

Quick links

Citations

2 citation in other articles

Structural basis for the ligand recognition and signaling of free fatty acid receptors.
Zhang et al. (2024)
1 more

1 mention without citation

Molecular mechanism of fatty acid activation of FFAR1.
Kumari et al. (2023)