› 8ejj

Electron Microscopy
3.22Å resolution

Lassa virus glycoprotein complex (Josiah) bound to S370.7 Fab

Released:
DOI: 10.2210/pdb8ejj/pdb
PDB entry 8ejj coloured by chain, front view.
PDB entry 8ejj coloured by chain, side view.
PDB entry 8ejj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies.
Perrett HR, Brouwer PJM, Hurtado J, Newby ML, Liu L, Müller-Kräuter H, Müller Aguirre S, Burger JA, Bouhuijs JH, Gibson G, Messmer T, Schieffelin JS, Antanasijevic A, Boons GJ, Strecker T, Crispin M, Sanders RW, Briney B, Ward AB
OpenAccess logo Cell Rep 42 112524 (2023)
PMID: 37209096
Related structures: EMD-28184

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235041 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (10 distinct):
Glycoprotein G1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 197 amino acids
Theoretical weight: 22.2 KDa
UniProt:
  • Canonical: P08669 (Residues: 59-255; Coverage: 40%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein
S370.7 heavy chain Fab Chains: H, I, h
Molecule details ›
Chains: H, I, h
Length: 128 amino acids
Theoretical weight: 14.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
S370.7 light chain Fab Chains: J, L, l
Molecule details ›
Chains: J, L, l
Length: 107 amino acids
Theoretical weight: 11.52 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Glycoprotein G2 Chains: a, b, c
Molecule details ›
Chains: a, b, c
Length: 164 amino acids
Theoretical weight: 19.15 KDa
UniProt:
  • Canonical: P08669 (Residues: 260-423; Coverage: 33%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 6 x NAG
Ligand MAN 1 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.22Å
Relevant EMDB volumes: EMD-28184
Expression system: Homo sapiens

Quick links

Citations

2 review citations

Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens.
Newby et al. (2024)
1 more