› 8ejo

X-ray diffraction
2.67Å resolution

Crystal structure of the homeodomain of Platypus sDUX in complex with DNA

Released:
DOI: 10.2210/pdb8ejo/pdb
PDB entry 8ejo coloured by chain, front view.
PDB entry 8ejo coloured by chain, side view.
PDB entry 8ejo coloured by chain, top view.
Source organisms:
Primary publication:
Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein.
Bosnakovski D, Toso EA, Ener ET, Gearhart MD, Yin L, Lüttmann FF, Magli A, Shi K, Kim J, Aihara H, Kyba M
OpenAccess logo iScience 26 107823 (2023)
PMID: 37744032

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-498337 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 71 amino acids
Theoretical weight: 8.3 KDa
Source organism: Ornithorhynchus anatinus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A6I8NF41 (Residues: 43-111; Coverage: 18%)
Sequence domains: Homeodomain
DNA (5'-D(*GP*CP*GP*TP*AP*AP*TP*CP*TP*AP*AP*TP*CP*AP*AP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.18 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*GP*TP*TP*GP*AP*TP*TP*AP*GP*AP*TP*TP*AP*CP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.23 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P43212
Unit cell:
a: 74.022Å b: 74.022Å c: 97.309Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.238
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

1 mention without citation

Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein.
Bosnakovski et al. (2023)