› 8eki

Electron Microscopy
4.5Å resolution

CryoEM structure of the Dsl1 complex bound to SNAREs Sec20 and Use1

Released:
DOI: 10.2210/pdb8eki/pdb
PDB entry 8eki coloured by chain, front view.
PDB entry 8eki coloured by chain, side view.
PDB entry 8eki coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of a membrane tethering complex incorporating multiple SNAREs.
DAmico KA, Stanton AE, Shirkey JD, Travis SM, Jeffrey PD, Hughson FM
Nat Struct Mol Biol (2024)
PMID: 38196032
Related structures: EMD-28204

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-230923 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Protein transport protein SEC20 Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 32.24 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28791 (Residues: 1-275; Coverage: 72%)
Gene names: D9719.4, SEC20, YDR498C
Sequence domains: Sec20
Protein transport protein USE1 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 24.25 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P53146 (Residues: 1-212; Coverage: 87%)
Gene names: SLT1, USE1, YGL098W
Sequence domains: Membrane fusion protein Use1
Protein transport protein TIP20 Chain: C
Protein transport protein SEC39 Chain: D
Protein transport protein DSL1 Chain: E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-28204
Expression system: Escherichia coli BL21

Quick links

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 8eki found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-11846
Datasets: 2
Total size: 9700Gb
EMPIAR thumbnail