› 8el7

Electron Microscopy
2.8Å resolution

CryoEM structure of Resistance to Inhibitors of Cholinesterase-8B (Ric-8B) in complex with G alpha s

Released:
DOI: 10.2210/pdb8el7/pdb
PDB entry 8el7 coloured by chain, front view.
PDB entry 8el7 coloured by chain, side view.
PDB entry 8el7 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of Ric-8B in complex with Gα protein folding clients reveal isoform specificity mechanisms.
Papasergi-Scott MM, Kwarcinski FE, Yu M, Panova O, Ovrutsky AM, Skiniotis G, Tall GG
Structure (2023)
PMID: 36931277
Related structures: EMD-28223

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215998 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Synembryn-B Chain: B
Molecule details ›
Chain: B
Length: 563 amino acids
Theoretical weight: 63.93 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q80XE1 (Residues: 1-483, 484-520; Coverage: 100%)
  • Best match: Q80XE1-1 (Residues: 1-560)
Gene name: Ric8b
Sequence domains: Guanine nucleotide exchange factor synembryn

Ligands and Environments

No bound ligands
2 modified residues:
Ligand SEP 1 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-28223
Expression system: Trichoplusia ni

Quick links

Citations

3 citation in other articles

A Zpr1 co-chaperone mediates folding of eukaryotic translation elongation factor 1A via a GTPase cycle.
McQuown et al. (2023)
2 more