› 8en1

X-ray diffraction
2.4Å resolution

Structure of GII.4 norovirus in complex with Nanobody 30

Released:
DOI: 10.2210/pdb8en1/pdb
PDB entry 8en1 coloured by chain, front view.
PDB entry 8en1 coloured by chain, side view.
PDB entry 8en1 coloured by chain, top view.
Source organisms:
Primary publication:
Direct Blockade of the Norovirus Histo-Blood Group Antigen Binding Pocket by Nanobodies.
Kher G, Sabin C, Lun JH, Devant JM, Ruoff K, Koromyslova AD, von Itzstein M, Pancera M, Hansman GS
J Virol 97 e0183322 (2023)
PMID: 36971561

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233137 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
VP1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.1 KDa
Source organism: Norovirus
Expression system: Escherichia coli
UniProt:
  • Canonical: K4LM89 (Residues: 224-540; Coverage: 59%)
Sequence domains: Calicivirus coat protein C-terminal
Nanobody 30 Chains: C, D
Molecule details ›
Chains: C, D
Length: 135 amino acids
Theoretical weight: 15.3 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Unit cell:
a: 117.767Å b: 62.443Å c: 121.396Å
α: 90° β: 101.24° γ: 90°
R-values:
R R work R free
0.21 0.207 0.264
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Direct Blockade of the Norovirus Histo-Blood Group Antigen Binding Pocket by Nanobodies.
Kher et al. (2023)